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M Flashner, P Wang, J B Hurley and S W Tanenbaum
The routine enzyme inducer was a hot-water extract of “edible bird’s nest.” Mild acid treatment (0.05 N H2SO4) of this extract increased enzyme activity
ABSTRACT
The elective isolation of a soil microorganism, tentatively assigned to the genus Arthrobacter, which produced an extracellular neuraminidase is described. The secretion of neuraminidase from washed cells in minimal medium required the presence of sialo-containing glycoproteins, whereas free N-acetyl-neuraminic asid of N-acetylmannosamine were poor inducers. No enzyme could be dected in the induction fitrated of cells, in the absence of inducer or in the culture filtrate of cells grown in a complete medium. The routine enzyme inducer was a hot-water extract of “edible bird’s nest.” Mild acid treatment (0.05 N H2SO4) of this extract increased enzyme activity two–to threefold and the specific activity about eightfold.
Read more about Properties of an inducible extracellular neuraminidase from an Arthrobacter isolate. …